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Innovative DNA Amplification
As a Molecular Diagnostic Techniques
Isothermal amplification techniques have been used to molecular diagnostic platforms with great success, negating the need for expensive thermal cyclers as need for qRT-PCR, which currently hinders point-of-care (POC) use. Several isothermal amplification systems have been reported over the past few years (e.g., LAMP, RPA, NASBA, RCA, HDA, EXPAR, etc.) and implemented in a POC test combined with nanoparticles.
We have recently developed a number of nanoparticle-based colorimetric or fluorescence assays capable of detecting and quantifying a panel of biomerkers (e.g., DNA, miRNA, enzymes, proteins). These include fluorescent quantum dot-based devices combined with isothermal DNA amplification strategies (e.g., tohold-mediated strand displacement, enzyme-assisted target recycling and signal amplification).
Here, we aim to employ a state of the art and synergistic approach to design a POC molecular diagnostic test that incorporates our unprecedentedly sensitive nanomaterial components and innovative DNA amplification techniques. The insights and technology we generate will provide the optimal plaform from which to take host gene response diagnosis from the lab bench to the field.
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